Activation of Human Phospholipase C-η2 by Gβγ
نویسندگان
چکیده
منابع مشابه
Synergistic Activation of Phospholipase C-β3 by Gαq and Gβγ Describes a Simple Two-State Coincidence Detector
BACKGROUND Receptors that couple to G(i) and G(q) often interact synergistically in cells to elicit cytosolic Ca(2+) transients that are several-fold higher than the sum of those driven by each receptor alone. Such synergism is commonly assumed to be complex, requiring regulatory interaction between components, multiple pathways, or multiple states of the target protein. RESULTS We show that ...
متن کاملA dynamic model of membrane-bound phospholipase Cβ2 activation by Gβγ subunits.
Phospholipase C (PLC) β2, a well studied member of the family of enzymes that catalyze the hydrolysis of the membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP₂) into secondary messengers, can be activated by the Gβγ subunits of heterotrimeric G-proteins in a manner that depends on the presence and composition of the associated phospholipid membrane surface. The N-terminal pleckstrin hom...
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Introduction One early transmembrane signal in response to stimulation of the T-cell antigen receptor (CD 3/Ti) is the activation of phosphatidylinositol (Ptd1ns)specific phospholipase C (PLC). The resulting formation of inositol 1,4,5-trisphosphate (Ins( 1,4,5)q?) and diacylglycerol (DAG) causes calcium release from internal stores, calcium influx through calcium channels, and translocation of...
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Phospholipase C 2 (PLC 2) has been implicated in collageninduced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLC 2. We expressed the full-length cDNA for human PLC 2 in bacteria and purified the recombinant enzyme. The recombinant enzyme was Ca -dependent with optimal activity in the range of 1 to 10 M C...
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Unique among the phospholipase C isozymes, the recently identified phospholipase C-epsilon (PLC-epsilon) contains an amino-terminal CDC25 domain capable of catalyzing nucleotide exchange on Ras family GTPases as well as a tandem array of Ras-associating (RA) domains near its carboxyl terminus that are effector binding sites for activated H-Ras and Rap. To determine whether other small GTPases a...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2008
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi800044n